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81.
Temporal and spatial variability in nearshore bacterioplankton communities of Lake Michigan 总被引:2,自引:0,他引:2
Sabrina R. Mueller-Spitz Giles W. Goetz & Sandra L. McLellan 《FEMS microbiology ecology》2009,67(3):511-522
The spatial and temporal variability of bacterial communities were determined for the nearshore waters of Lake Michigan, an oligotrophic freshwater inland sea. A freshwater estuary and nearshore sites were compared six times during 2006 using denaturing gradient gel electrophoresis (DGGE). Bacterial composition clustered by individual site and date rather than by depth. Seven 16S rRNA gene clone libraries were constructed, yielding 2717 bacterial sequences. Spatial variability was detected among the DGGE banding patterns and supported by clone library composition. The clone libraries from deep waters and the estuary environment revealed highest overall bacterial diversity. Betaproteobacteria sequence types were the most dominant taxa, comprising 40.2–67.7% of the clone libraries. BAL 47 was the most abundant freshwater cluster of Betaproteobacteria , indicating widespread distribution of this cluster in the nearshore waters of Lake Michigan. Incertae sedis 5 and Oxalobacteraceae sequence types were prevalent in each clone library, displaying more diversity than previously described in other freshwater environments. Among the Oxalobacteraceae sequences, a globally distributed freshwater cluster was determined. The nearshore waters of Lake Michigan are a dynamic environment that experience forces similar to the coastal ocean environment and share common bacterial diversity with other freshwater habitats. 相似文献
82.
青海湖鸟岛斑头雁种群对H5N1亚型禽流感病毒的免疫状况 总被引:1,自引:0,他引:1
斑头雁(Anser indicus)是2005年青海湖H5N1型高致病性禽流感的主要被感染物种。为了解斑头雁目前对H5N1亚型禽流感病毒(AIV)免疫状况,2008年春季,在青海湖鸟岛采集该种群弃卵(68枚)和巢卵(125枚),以血凝抑制试验(HI)检测抗H5N1亚型禽流感病毒的卵黄母源抗体(IgY)。根据测试结果推断,在高致病性禽流感暴发3年后,青海湖鸟岛繁殖的斑头雁种群有26.5%~35.2%的繁殖对可能已经获得了对H5N1型禽流感病毒的免疫能力。另外,以斑头雁巢密度和抗体效价进行相关分析发现,斑头雁母源抗体水平与斑头雁巢密度正相关(r=0.736, P=0.000),表明高密度繁殖群内的母源抗体传递更具有适应性意义。 相似文献
83.
滇池金线鲃形态度量学分析(Cypriniformes:Cyprinidae) 总被引:2,自引:0,他引:2
运用多变量形态度量学和框架结构形态学的方法,测量了采自滇池流域6个不同地点的149尾滇池金线鲃(Sinocyclocheilus grahami)的标本。应用多变量形态度量学分析,选择了20个框架结构性状和19个常规性状,进行主成成分分析。主成成分分析结果的散布图显示,6个不同地点的标本聚在一起没有分开,表明滇池金线鲃的各居群之间在可数性状和可量性状上均未表现出明显的差异,说明滇池金线鲃在形态上还未发生明显的种下分化。 相似文献
84.
85.
A large-scale enclosure experiment for lake restoration was carried out in Lake Wuli, a northern bay of shallow and eutrophic Lake Taihu in China. The large enclosure with an area of 10 ha was set up in the littoral zone and was bordered by waterproof fabric which did not cover the sediments. Multiple approaches were used and included fish removal, piscivorous fish stocking, shoreline reconstruction, aquatic macrophyte planting, benthic macro-animal stocking, and silver carp cultivation in pens for reduction of cyanobacteria. The results showed that the coverage of aquatic macrophytes increased from 0% to 45.7%. Mean concentrations of TN and TP inside the enclosure from May 2004 to May 2008 were 22.2% and 26.0% of those outside, respectively. Secchi depth was 0.40 m outside the enclosures and 0.75 m inside. However, responses of phytoplankton to the restoration project lagged behind improvement of water quality and reestablishment of aquatic plants. The phytoplankton biomass gradually decreased after the third year of the restoration. Stocking piscivorous fish and planting submerged macrophytes could not increase zooplankton biomass and enhance graze pressure on phytoplankton, most likely due to high omnivorous fish density and lower nutrition inside the enclosure. Higher grazing pressure of zooplankton on phytoplankton was observed in May and October every year. Zooplankton to phytoplankton biomass ratios were significantly negatively correlated with phytoplankton biomass outside (r = −0.440, p < 0.01) and inside the enclosure (r = −0.336, p < 0.05) from February 2004 to March 2007. Therefore, phytoplankton biomass inside and outside the enclosure was lower in May and October. Higher grazing pressure of zooplankton on phytoplankton in spring may result in occurrence of the clear-water phase that facilitated growth of submerged macrophytes in the littoral in Lake Wuli, and a clear-water state and improved water quality would likely be sustained throughout the year after reestablishment of submerged macrophytes. 相似文献
86.
Dennis Trolle Guangwei Zhu David Hamilton Liancong Luo Chris McBride Lu Zhang 《Hydrobiologia》2009,627(1):31-44
Distinct horizontal water column concentration gradients of nutrients and chlorophyll a (Chl a) occur within large, shallow, eutrophic Lake Taihu, China. Concentrations are high in the north, where some of the major
polluted tributaries enter the lake, and relatively low in the south, where macrophytes generally are abundant. It is not
clear, however, whether these water column concentration gradients are similarly reflected in spatial heterogeneity of nutrient
concentrations within the bottom sediments. The main objective of this study was therefore to test if horizontal and vertical
variations in the phosphorus and nitrogen content in bottom sediments of Lake Taihu are significantly related to (1) horizontal
variations in overlying water column nutrient concentrations and (2) other sediment geochemical constituents. We measured
the concentration of total phosphorus (TP) and total nitrogen (TN) in surficial sediments (0–2 cm) and TP, TN and Chl a concentrations in water column samples, collected from 32 sites in 2005. In 2006 sediment, TP, TN, carbon, iron and manganese
concentrations were measured vertically at 2 cm intervals, extending to a depth of approximately 20 cm, at an additional eight
sites. Linear correlation analysis revealed that surficial sediment TP concentrations across the 32 stations were related
significantly, though weakly, to annual mean water column concentrations of TP, TN as well as Chl a. Correlations of surficial sediment TN with water column variables were, however, not significant (P > 0.05). Amongst the geochemical variables tested, the vertical variability of sediment TP concentrations was most strongly
related to sediment manganese and carbon concentrations. A multiple stepwise linear regression revealed that the combination
of sediment manganese and carbon concentrations explained 91% of the horizontal variability in sediment TP concentrations
and 65% of the vertical variability.
Handling editor: Luigi Naselli-Flores 相似文献
87.
Site-specific chlorophyll reference conditions for lakes in Northern and Western Europe 总被引:1,自引:1,他引:0
Laurence Carvalho Angelo G. Solimini Geoff Phillips Olli-Pekka Pietiläinen Jannicke Moe Ana Cristina Cardoso Anne Lyche Solheim Ingmar Ott Martin Søndergaard Gianni Tartari Seppo Rekolainen 《Hydrobiologia》2009,633(1):59-66
The Water Framework Directive (WFD) requires EU Member States to assess the “ecological status” of surface waters. As a component of ecological status, many European countries are developing a classification scheme for chlorophyll concentrations as a measure of phytoplankton biomass. The chlorophyll classification must be based on the degree of divergence of a water body from an appropriate baseline or ‘reference condition’. This article describes the development of a series of regression models for predicting reference chlorophyll concentrations on a site-specific basis. For model development, a large dataset of European lakes considered to be in reference condition, 466 lakes in total, was assembled. Data were included from 12 European countries, but lakes from Northern and Western Europe dominated and made up 92% of all reference lakes. Data have been collated on chlorophyll concentration, altitude, mean depth, alkalinity, humic type, surface area and geographical region. Regression models were developed for estimating site-specific reference chlorophyll concentrations from significant predictor ‘typology’ variables. Reference chlorophyll concentrations were found to vary along a number of environmental gradients. Concentrations increased with colour and alkalinity and decreased with lake depth and altitude. Forward selection was used to identify independent explanatory variables in regression models for predicting site-specific reference chlorophyll concentrations. Depth was selected as an explanatory variable in all models. Alkalinity was included in models for low colour and humic lakes and altitude was included in models for low colour and very humic lakes. Uncertainty in the models was quite high and arises from errors in the data used to develop the models (including natural temporal and spatial variability in data) and also from additional explanatory variables not considered in the models, particularly nutrient concentrations, retention time and grazing. Despite these uncertainties, site-specific reference conditions are still recommended in preference to type-specific reference conditions, as they use the individual characteristics of a site known to influence phytoplankton biomass, rather than adopt standards set to generally represent a large population of lakes of a particular type. For this reason, site-specific reference conditions should result in reduced error in ecological status classifications, particularly for lakes close to typology boundaries. 相似文献
88.
Wolfgang Nikolaus Probst Stefan Stoll Lars Peters Philipp Fischer Reiner Eckmann 《Hydrobiologia》2009,632(1):211-224
In Lake Constance, Eurasian bream Abramis brama (L.) spawn in very shallow littoral areas by the beginning of May. They attach their adhesive eggs to pebble and cobble substratum
at <40 cm depth. Increasing water levels before spawning inundate bare substratum to which bream eggs may attach better than
to deeper substratum covered by epilithon. Consequently, the water level increase prior to spawning should determine the amount
of pristine spawning substratum available to bream and thus influence their breeding success. In order to test this hypothesis,
the influence of hydrology and climate on the abundance of age-0 bream was combined with the results from field investigations
on the egg survival and abundance of age-0 bream. A strong positive correlation between the mean water level increase during
the spawning season of bream (April–May) and the abundance of juvenile bream was found. In contrast, the absolute water level
during spawning and during the nursery stage in summer, the cumulative temperature during the egg, larval and juvenile stages
and two North Atlantic Oscillation (NAO) indices did not affect the abundance of juvenile bream. The field investigations
confirmed that bream eggs attach better to and have higher survival rates on bare substratum than on substratum with epilithon
cover. Accordingly, eggs within a spawning habitat of bream were most abundant between 10 and 20 cm depth, where the epilithon
cover was lower than at depths exceeding 30 cm. The results of this study confirm an adverse influence of epilithon cover
on the attachment and subsequent survival of bream eggs and emphasize the importance of spring inundations for the successful
breeding of the bream.
Handling editor: J. A. Cambray 相似文献
89.
Sébastien Personnic Isabelle Domaizon Ursula Dorigo Lyria Berdjeb Stéphan Jacquet 《Hydrobiologia》2009,627(1):99-116
Flow cytometry (FCM) was used to assess microbial community abundances and patterns in three natural, large and deep peri-alpine
hydrosystems, i.e., lakes Annecy (oligotrophic), Bourget, and Geneva (mesotrophic). Picocyanobacteria, small eukaryotic autotrophs,
heterotrophic prokaryotes, and viruses were studied in the 0–50 m surface layers to highlight the impact of both physical
and chemical parameters as well as possible biotic interactions on the functioning of microbial communities. Some specificities
were recorded according to the trophic status of each ecosystem such as the higher number of viruses and heterotrophic bacteria
in mesotrophic environments (i.e., Lakes Geneva and Bourget) or the higher abundance of picocyanobacteria in the oligotrophic
Lake Annecy. However, both seasonal (temperature) and spatial (depth) variations were comparatively more important than the
trophic status in driving the microbial communities’ abundances in these three lakes, as revealed by principal component analysis
(PCA). A strong viral termination of the heterotrophic bacterial blooms could be observed in autumn for each lake, in parallel
to the mixing of the upper lit layers. As virus to bacteria ratio (VBR) was indeed very high at this period with values varying
between 87 and 114, such important relationships between viruses and bacteria were likely. The magnitudes of seasonal variations
in VBR, with the highest values ever reported so far, were largely greater than the magnitude of theoretical variations due
to the trophic status, suggesting also a strong seasonality in virioplankton production associated to prokaryotic dynamics.
FCM analyses allowed discriminating several viral groups. Virus-Like Particles group 1 (VLP1) and group 2 (VLP2) were always
observed and significantly correlated to bacteria for the former and chlorophyll a and picocyanobacteria for the latter, suggesting that most of VLP1 and VLP2 could be bacteriophages and cyanophages, respectively.
On the basis of these results, new ways of investigation emerge concerning the study of relationships between specific picoplanktonic
groups; and overall these results provide new evidence of the necessity to consider further viruses for a better understanding
of lake plankton ecology.
Handling editor: Luigi Naselli-Flores 相似文献
90.
Lake Victoria, the world's largest tropical freshwater lake, is an important resource, ecologically and economically. THg distribution in the northern parts of the lake are not well known, so to answer this gap, patterns in total mercury (THg) in water, soil and two dated sediment cores from northern Lake Victoria were determined. Water THg concentrations ranged from 0.7 to 5.8 ng/L, and there were no apparent differences observed between Napoleon and Winam Gulfs. Two precipitation samples had Hg concentrations of 7 and 31 ng/L. Surface soil samples collected from various agricultural sites around Jinja, Napoleon Gulf, have THg concentrations between 12.7 and 48.4 ng/g dry weight; they were correlated with organic carbon, total phosphorus and % clay. A near-shore core taken in Itome Bay in Napoleon Gulf, and an offshore core collected from the deepest part of the lake had similar THg concentrations and profiles (78 to 458 ng/g dry weight). The increase in THg concentration in the profiles of both cores began around 1960 and peaked around 1980. The similar sedimentary THg profiles and fluxes in the cores suggest that the THg sources to L. Victoria are primarily atmospheric, with some erosion inputs, and that equatorial African ecosystems are not exempt from the global increase in baseline THg concentrations. 相似文献